Retinol palmitate and retinol acetate in formulas for special medical purposes intended for infants was determined using NP-HPLC. The HPLC system consisted of NH₂ column (150 mm×4.6 mm, 5.0 μm), mobile phase of n-hexane-25.0% tert-butyl methyl ether n-hexane solution (955), flow rate of 1.0 mL/min, injection volume of 50 μL, column temperature of 30 ℃, UV detector detection wavelength of 325 nm. Results showed that retention time of retinol palmitate and retinol acetate were 3.5 min and 6.0 min, respecitively the linearity was good in the range of 0.05～10.0 μg/mL with a correlation coefficient of 0.999 9, the detection limits were 2.0 and 2.5 μg/100 g respectively. The content of retinol palmitate and retinol acetate in formulas for special medical purposes intended for infants were 0～817.0 and 0～8 683.0 μg/100 g, the precision were 0.9%～9.4% and 3.3%～6.3% respecitively. The recoveries of spiked samples at three levels were 81.0%～90.0%. The developed method presented the benefits of simplicity, rapidity, good separation, high accuracy, high precision.