Zeta potential analyzer, conductivity meter and electrochemical workstation were used to analyze the variation of zeta potential, electrical conductivity and redox potential, with addition of different divalent metal ions (Ca²⁺, Ba²⁺, Mn²⁺ and Cu²⁺) to ovalbumin solution before and after pulsed electric field treatment. The results showed that: ① the zeta potential of ovalbumin solution with Ba²⁺ and Cu²⁺ decreased with the addition of pulsed electric field energy and samples with Ca²⁺ and Mn²⁺ increased first and then decreased; ② With the increase of pulse electric field treatment time, the conductivity of protein solution with metal ions increased first and then decreased, among which Ca²⁺ had the greatest effect on the conductivity of protein solution; ③ the electrical activity and quantity of conductive materials decreased with the addition of four metal ions. Besides, the values of redox potential reduced gradually with increasing the treatment times.