In order to improve the accuracy and repeatability of the integration quantitative determination method based on ImageJ for the ash from the burning cigarette., an optimized scheme was conducted in this study. The scheme was optimized from three aspects, including the selection of cigarette combustion surface, the selected height as well as the width of the burning cigarette columnar ash. The established optimal scheme was as follows: ① Taking pictures of the burning cigarette when it was burned to 1 cm away from the tipping paper. ② Using a height of 25 mm, 6.46 mm width rectangular box to select the bottom of the gray column as a starting point for analysis of the picture area. ③ The final result of the ash integration was taken from the average of three cigarettes and three burning surfaces (the surface, opposite face and the side face of lapped cigarette paper ). This program could weaken the influence of the lapped cigarette paper, the curvature of the ash as well as the outer ash on the repeatability of measurement results. In addition, this optimized method could reflect the true ash integration character more comprehensively. The application of this method indicated that the RSD of the four samples reduced from 26.18% ~ 34.52% to 15.3% ~ 20.6%, suggesting a significant improvement in accuracy and repeatability.