Based on the results of single-factor experiments, orthogonal test of ursolic acid from Amygdalus communis L. peel were carried out to determine the optimum process parameters.The antioxidant activities were evaluated by the reducing power, DPPH free radical, hydroxyl free radical and hemolysis of mice red blood cells.The optimum extraction parameters were determined as follows: alcohol concentration 65%,extraction temperature 80 ℃; solid-to-solvent ratio 120 (g/mL); extraction time 1.5 h. Under these conditions, the experimental yield of ursolic acid was 2.89%. The concentration of ursolic acid was 0.1 mg/ml, ursolic acid of Amygdalus communis L. peel displayed a strong antioxidant activity, the percentage scavenging of DPPH free radical and ·OH were 87.87% and 62.11%, respectively.The concentration of ursolic acid was 0.6 mg/mL ,the oxidation of mice red blood cell hemolysis inhibition rate was 97.83%.