Abstract:The purple bud tea zijuan,zixuan9803,Hongyafoshou an An73 shoots were used as research objects. Using three plant anthocyanins as external standards,a HPLC method for simultanteous detection of various anthocyanins in purple bud tea tree was established. That is: after been extracted with 80% ethanol and refluxed, the tea is degreased with a volume ratio of 112 mixture of n-hexane:ethyl acetate:water. A C18 column was used with acetonitrile and 0.2% phosphoric acid ultra-pure water as the mobile phase. Gradient,the organic phase elution gradient: 0~55 min, 10%~40%, flow rate 0.8 mL/min, column temperature 30 ℃, detection wavelength 520 nm. Each anthocyanins can be effectively separated, and the correlation coefficient of the standard curve is ≥0.99, the linear range is 5.00~75.00 μg/L. The precision and repeatability of the method is good. The relative standard deviation of the stability≤10%, the recovery rate is 75%~88%.Seven major anthocyanin components in four kinds of purple bud tea samples are effectively separated. The method has the characteristics of rapidity, sensitivity, accuracy, and low cost. It is also an analysis method which can be used to simultaneously detect and analyze various anthocyanin components in the purple bud tea.