Establishment of HPLC method for anthocyanins determination of purple bud tea tree
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(1. Key Lab of Tea Science of Ministry of Education, Changsha, Hunan 410128, China; 2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128,China; 3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China)

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    Abstract:

    The purple bud tea zijuan,zixuan9803,Hongyafoshou an An73 shoots were used as research objects. Using three plant anthocyanins as external standards,a HPLC method for simultanteous detection of various anthocyanins in purple bud tea tree was established. That is: after been extracted with 80% ethanol and refluxed, the tea is degreased with a volume ratio of 112 mixture of n-hexane:ethyl acetate:water. A C18 column was used with acetonitrile and 0.2% phosphoric acid ultra-pure water as the mobile phase. Gradient,the organic phase elution gradient: 0~55 min, 10%~40%, flow rate 0.8 mL/min, column temperature 30 ℃, detection wavelength 520 nm. Each anthocyanins can be effectively separated, and the correlation coefficient of the standard curve is ≥0.99, the linear range is 5.00~75.00 μg/L. The precision and repeatability of the method is good. The relative standard deviation of the stability≤10%, the recovery rate is 75%~88%.Seven major anthocyanin components in four kinds of purple bud tea samples are effectively separated. The method has the characteristics of rapidity, sensitivity, accuracy, and low cost. It is also an analysis method which can be used to simultaneously detect and analyze various anthocyanin components in the purple bud tea.

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刘林峰,周阳,林玲,等.茶树紫芽中多种花色苷组分同时检测方法的建立[J].食品与机械英文版,2018,34(2):67-72.

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  • Received:January 15,2018
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  • Online: March 17,2023
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