It showed that soybean peptides and taurine had good anti-fatigue effects. In order to explore the function of soybean peptide and taurine compound powder (SPT) on relieving fatigue and the interaction between them, the SPT Compound powder was used to test the SPF KM mice, and groups high, medium and low doses and a distilled water blank control fed for continuous 30 days were set. The experiments of loaded swimming, blood lactic acid, serum urea, liver glycogen determinations were then detected. The results showed that with the increase of the dosage, the loaded swimming time increased significantly. However, the area of blood lactic acid was decreased significantly. The serum urea was also decreased without statistical significance between the dose groups and the control. The content of liver glycogen increased significantly as well. According to “Technical Standards for Testing &Assessment of Health Food (2003)”, SPT Compound powder was deemed to have the function of relieving physical fatigue.