The pigments were obtained from leaves of Ginkgo biloba L. using ultrasound-assisted ethanol extraction method. The main influence factors of pigments extraction were selected from the results of single factor test and optimized by orthogonal experimental design, and its stability to pH, temperature, metal ions and light was studied. The optimum extraction technology was using the ethanol concentration of 95%, with the pH value 6.0, extracted at 60 ℃ for 2 h. Under this condition, the absorbance of pigments was 0.831. The stability results indicated that the pigments was stable in acidic and alkine media, and was stable to Na⁺ and Ca²⁺, but unstable to Fe³⁺, Cu²⁺ and Mg²⁺ relatively. It would accelerate the pigments decomposition when the temperature was higher than 70 ℃, and it had instability to the sunlight and indoor light, so it should be stored away from light.