The active ingredients of crude propolis from Yongzhou were extracted by graded ethanol method and investigated the scope of their activity. With flavonoids content as index, content and the antioxidant activity of them in the crude propolis from different extraction conditions was evaluated. The results showed that the quality and content of flavonoids in the crude propolis extracted with 40%～60% ethanol were higher. The content of flavonoids in the extracts by graded ethanol method reached 41.2% and the extraction yield of flavonoids was 25.81%. The antioxidant activity of flavonoids of crude propolis extracted with 50% and 60% ethanol were better than Vc, but the one in the crude propolis extracted with 30% ethanol was the worst. It illustrated the crude propolis contained different types of flavonoids. To obtain high active flavonoids of crude propolis, water should be chosen as extraction solvent to water-soluble flavonoids and 40%～70% ethanol should be chosen as extraction solvent to medium polarity flavonoids.