The physicochemical characteristics and digestibility of native starch from Dioscorea opposite Thunb. cv. Huaiqing and its resistant starch produced by autoclave process preparation method have been analyzed and compared in the paper. Results showed that the shape of native starch granules from Dioscorea opposite Thunb. cv. Huaiqing was irregular ellipsoid and triangle with a smooth surface. The crystal form of naitive starch was type C. Resistant starch losing particle characteristic exhibited a lamellar structure with loose surface. Both of them were similar in chemical structure without new functional group in resistant starch granules. Compared with the native starch, the molecular weight distribution of resistant starch was more concentrated. The peak temperature of resistant starch gelatinization was higher than that of naitive starch, and the resistant starch had better thermal stability. The transparency of resistant starch was lower than naitive starch. When bath temperature was lower than 75 ℃, the water-holding capacity of resistant starch was greater than that of naitive starch, and while bath temperature was above the gelatinization temperature of native starch, water-holding capacity of naitive starch, which was much higher than that of resistant starch. The results of simulating human digestion based on In-Vitro mode showed that resistant starch was more resistant to digestion than naitive starch.