To evaluate the antioxidant, whitening effect and skin safety of rose fermented broth. Method: Rose fermented broth was acquired by fermenting saccharomyces cerevisiae with rose. The skin safety, antioxidant effect and whitening effect were evaluated. The human whole gene expression profile chip was used to explore the whitening mechanism of rose fermented broth. The results showed that the scavenging effect of rose fermented broth on DPPH radical scavenging was obvious and its inhibitory effect on the activity of extracellular tyrosinase was in direct proportional to the concentration. The intracellular tests showed that rose broth had inhibitory effect on tyrosinase activity and melanin synthesis. The gene chip detection showed that the possible mechanism of whitening was inhibiting the growth of melanoma cells by down regulating the gene expressions of NGF and FGF2 in the melanoma pathway. Human patch test showed that none positive reaction was caused by rose fermented broth. Conclusion: the rose fermented broth had a high skin safety as well as antioxidant and whitening effect. The whitening effect might be caused by down regulation of NGF and FGF2.