The thermophysical properties of tobacco and smoke agent (the mass ratio of glycerol and propylene glycol is 51) was measured by transient plane heat source method, and the effects of moisture and smoke agent content on reconstituted cut tobacco and flue-cured were measured. The results showed that with the increase of temperature (22～75 ℃), thermal conductivity \[0.06～0.12 W/(m·K) and 0.06～0.16 W/(m·K\] and volume heat capacity \[0.17～2.70 MJ/(m³K) and 0.15～1.45 MJ/(m³K)\] increased; however, the thermal diffusivity decreased (0.33～0.05 mm² / s and 0.40～0.09 mm² / s, respectively). With the increase of moisture content (0%～13%) , the thermal conductivity and the volume heat capacity of tobacco materials increased, however, the thermal diffusivity decreased. With the increase of smoke agent content (0%～25%), the thermal conductivity of tobacco material \[0.005 W/(m·K) and 0.006 W/(m·K)\], the volume heat \[0.106 MJ/(m³K) and 0.138 MJ/(m³K)\] increased slightly, but the thermal diffusivity decreased ( 0.069 mm² / s and 0.069 mm² / s, respectively).