The sea-buckthorn seed oil microcapsules were prepared by spray drying technique with three kinds of recipes (CAS/MD, OSA/MD, WPI/GA/MD). The properties and microstructure of sea-buckthorn seed oil microcapsules were studied. Moreover, the content of fatty acid in sea-buckthorn seed oil before and after microencapsula-tion was determined by gas chromatography. The results showed that the microcapsules of the three formulations had low water content and high solubility. The encapsulation efficiency of sea-buckthorn seed oil microcapsule prepared with CAS/MD as wall material was highest. The sea-buckthorn seed oil microcapsules had small particle size, smooth surface, good thermal stability and uniform wall thickness. The distribution of seabuckthorn seed oil in capsules was uniform. The process of spray drying did not affect the functional components in sea-buckthorn seed oil.