In this paper, the application and optimum adding dosage of calcium carbonate, diatomite and titanium dioxide with different crystal form and particle size distribution on reconstituted tobacco sheet were investigated, and the effects of adding dosage on the physical properties of the reconstituted tobacco were discussed. The difference of filler retention on reconstituted tobacco was compared by the SEM and TG. The results showed that: the retention of diatomite and titanium dioxide was obviously higher than calcium carbonate, and the bulk and thickness were improved with diatomite and titanium dioxide; the tensile strength was highest at adding dosage of 10% when calcium carbonate or diatomite was used, and it changed little when titanium dioxide was used; the SEM shows that the retention of fillers was increasing with the increasing fillers ratio, and the calcium carbonate distribution was dispersed; in the TG analysis， the residual weight of reconstituted tobacco was in accordance with retention of fillers; the sensory quality of reconstituted tobacco of filling diatomite and titanium dioxide was better than filling calcium carbonate.