Objective To prepare a new kind of grease resistant -antibacterial paper using natural biological material as grease resistant and antibacterial agents, and to test its grease resistance and antibacterial properties. Methods Grease resistant-antibacterial paper was prepared by coating grease resistant antibacterial agent on paper. The grease resistance grade was tested by surface repellency test. The inhibition rate of coated paper on Escherichia coli and Staphylococcus aureus was calculated by colony method. Results When the ratio of 1 g/100 mL chitosan to 10 g/100 mL starch was 12, the grease resistance grade of coated paper reached 8, the cost was about 0.3 RMB/m²; when the addition amount of Nisin reached 1 g/100 mL, the inhibitory rates of coated paper on Escherichia coli and Staphylococcus aureus were 70.3% and 72.8%, respectively. Conclusion Nisin-loaded chitosan -starch coated paper, while ensuring grease resistance of paper, cost less than chitosan coated paper and has more suitable viscosity than chitosan and starch. It also has better toughness and flatness than starch coated paper, and can inhibit Staphylococcus aureus and Escherichia coli effectively.