In order to improve the efficient secretion of intracellular product in Saccharomyces cerevisiae, wild diploid Y₁ (2n) were mutagenized with UV treatment as original strain. To further identify, the degree of autolysis of each mutant strain was contrasted in low permeability condition by the test of its content of FDP, extra-cellular nucleic acids and proteins. The results showed: Hs₅*（n）showed the highest degree of conditional autolysis in low permeability condition, the concentration of FDP in extracellular environment was 25.81 μg/mL. At the sixth hour in low permeability, its penetration rate of nucleic acid and protein in extracellular environment were 1.86 and 2.07, respectively. In addition, mutant strain Hs₂*（n）, Hs₁（2n）also had a great extent of conditional autolysis capacity. These three low-permeability mutant strains obtained in this study possessed greater efficiency of intracellular product secretion contrast with wild strain.