Purification of tea saponin with macroporous resin and the changes of properties of the product were investigated. With comparisons of eight kinds of macroporous resin with different polarity to the crude tea saponin through static adsorption and dynamic adsorption, XR910X resin was selected to refine the tea saponin. Moreover, it was found that the tea saponin adsorption by XR910X would be matched by Freundlich Model. The optimal purification conditions were as following: the loading sample was crude tea saponin solution with pH 6.0, and the loading volume 1 BV, loading velocity 1.71 BV/h. Then 0.05 g/100 mL NaOH solution was used to remove impurity with 1.71 BV/h velocity and 2 BV volume. At last, 90% ethanol of 2 BV volume was used as eluent to desorb the tea saponin with the ethanol flow rate 1.71 BV/h. Under that condition, the recovery rate of tea saponin was 70.34% and the purity was 94.26%. In addition, the properties of tea saponin after purification in face activity and antioxidant capacity as well as antibacterial activities were much higher than those before purification.