The effects of pulsed electric field on chelating of ovalbumin and metal ions (Cu²⁺, Ba²⁺, Mn²⁺ or Ca²⁺) and the effect of pulsed electric field on the chelating effect of ovalbumin were studied by Raman spectroscopy. Results: ① PEF treatment time and metal ion species could affect the chelation of metal ions and protein, and the chelation of metal ions with ovalbumin was enhanced with the prolongation of pulse treatment time, when PEF treatment time was 1 695 μs, the chelation of Mn²⁺ and Cu²⁺ with protein began to weaken; ② The protein and metal ions chelated to different degrees at 1 200～1 700 cm^-1, the strongest was Mn²⁺ , followed by Cu²⁺ and Ca²⁺, and the weakest was Ba²⁺.