In order to increase the production of the antimicrobial substances by P.bovis BD3526 in wheat bran broth，the effect of individual fermentation parameter including cultivation temperature, period, inoculum ratio, age of the inoculum and concentration of wheat bran on the inhibitory intensity of the fermented wheat bran broth was determined, employing Micrococcus luteus as the indicator. Based on the results of single factor tests, four variables i.e. cultivation temperature, cultivation period, inoculum ratio and concentration of wheat bran were employed to further optimize the fermentation conditions for antimicrobial components production by the strain BD3526 by response surface methodology. The optimal cultivation conditions for BD3526 to produce antimicrobial substance were composed of a cultivation temperature of 29 ℃, a cultivation period of 73 h, an inoculum ratio of 3.2% and a wheat bran concentration of 4.2%. The inhibitory zone of the cultivated wheat bran broth under the optimal condition on M.luteus reached 19.88 mm in diameter, and the potency of bacteriocin produced under the optimal conditions was raised by 100% and 300% as compared to that produced under the original fermentation conditions.