The effect of total flavonoids from Perennial Lablab sp. Hull(FPLH) on the immune function and antioxidant activity in vivo were studied. With index of immune organ, spleen lymphocyte proliferation function, phagocytic activity of macrophages, cytokine levels in serum(IFN-γ and IL-4), the activity of alkaline phosphatase in serum, the activity of SOD, GSH-Px and the level of MDA in serum as the evaluated indices, the immune function and antioxidant activities of FPLH in mice was studied. The results showed that the thymus index and spleen index of FPLH was higher than the control group by 12.34% and 8.67% respectively; the spleen lymphocyte proliferation activity, macrophage phagocytic activity, IFN-γ, AKP, SOD and GSH-Px levels in serum were significantly increased by 114.68%, 27.16%, 8.25%, 82.52%, 10.47%and 12.16% respectively; the MDA level in serum was significantly decreased by 32.97%. FPLH showed strong immune function and antioxidant activity in vivo.