In order to explore the feasibility of the application of new fluorine free refrigerant in the existing refrigeration system, a traditional one based on R600a refrigeration, a testing equipment of new refrigeration system was designed. This new testing equipment was dominated by a cooling equipment, temperature tester and pressure detector. In this study, the refrigerant R600a replaced by a new type of fluorine free refrigerant HCR22, and the coefficient of performance and cold storage capacity were taken as indexes, and conducted an experiment for 12 h, the refrigerating capacity of two refrigerants in different quantity of charging ratio was studied. The results indicated that compared to R600a, the best filling ratio of HCR22 was less 13.33%, but the refrigeration coefficient was higher 10.53%, and the system energy was less 3.16%. When the refrigeration system was run for 12 h, the refrigeration ability of HCR22 was found better, and the cooling ability of HCR22 and R600a were 7 306.64 kJ and 6 796.68 kJ, respectively. This study provided evidences and technical support for the new type refrigerant HCR22 replacing the R600a refrigerant.