Compared to single temperature zones refrigerated truck, multiple temperature zones refrigerated truck is more comfortable with cargo demand and temperature changes. Air distribution in the truck cold room could affect its temperature significantly. In this paper, the influence of air distribution to temperature range in single evaporator multiple temperature zones refrigeration truck was studied based on CFD technology. Moreover, temperature non-uniformity problems were also analyzed. The results provided a theory basis for the design optimization of multiple zones refrigerated truck.