High pressure carbon dioxide treatment (HPCD) and ultra-high pressure homogenization treatment (HPH) are promising non-thermal sterilization technologies. A new type of combining sterilization technology of HPCD and HPH was developed to enhance the sterilization capabilities. The working principles, characteristics, structural components of the combining sterilization technologies were introduced systematically, and the physical prototype was developed. These three sterilization technologies were evaluated by comparing the inactivation of Saccharomyces cerevisiae, and the test results showed that the combining sterilization technology was better than that of the single one.