In this paper, the type II diabetes model was established and the optimal dose of the pumpkin polysaccharide, pueraria flavonoids and pu-erh theabrowns were determined by measured blood glucose. On the basis, the fasting blood glucose (FBG), insulin (INS), insulin resistance (IR), high density lipoprotein (HDL) and low density lipoprotein (LDL) were also investigated. The FBG, INS, IR, HDL, LDL combined group was significantly better than the single component (P＜0.05). The results revealed that the combinations of pumpkin polysaccharide and pu-erh theabrowns, pueraria flavonoids and pu-erh theabrowns were effective. However, the pumpkin polysaccharide factorial analyses showed that the interactions of the three substances were interactive. The synergistic experiment showed that pumpkin polysaccharide combined with Pueraria lobata flavonoids or combination with tea-flavonoid significantly reduced TG content (P＜0.05). The combination of theaflavin and pueraria flavonoids in reducing the content of FBG, TC, LDL was significantly better than flavonoids, and the effect of TG and INS was better than that of thepueraria flavonoids and pu-erh theabrowns group (P＜0.05). The molecular mechanism experiments showed that a single one and two of the three components played an important role in the expression of PI3K / Akt protein pathway.