To study the detoxification effect of electron beam irradiation on AFB₁ in corn, the effect of electron beam irradiation on the AFB₁-contaminated corn based on the doses of 0~50 kGy was studied. Physicochemical properties including fatty acid, color and viscosity of AFB₁-contaminted corn at different irradiated doses were also investigated. Results indicated that AFB₁ in corn significantly decreased with the irradiation dose (P<0.05). Moreover, physicochemical properties changed significantly when the irradiation dose was over 15 kGy, including the significant increase of fatty acid value (P<0.05) and the decrease of the viscosities (P<0.05). It was found that the whiteness didn’t change significantly (P>0.05), while the significantly decreased redness and yellowness (P<0.05) were observed.