\[Objective\] Response surface methodology was used to optimize the extraction process condition of polyphenols from red-flesh apple with ultrasonic assistance. \[Method\] Using the red-flesh apple of XINJIANG as material, on the basis of single-factor experiment, four factors, inclouding extraction time, ultrasonic power, extraction temperature and ratio of material-to-liquid were chosed to design the experiment with response surface method. With Box-Behnken design and response surface analysis, the optimum extracting conditions of polyphenols from red-flesh apple were determined. \[Result\] The optimal process parameters of polyphenol extraction were determined and they were as follows: extraction time and temperature were 20 min and 62 ℃, ultrasound power and ratio of material-to-liquid was 360 W and 14(g/mL) respectively. \[Conclusion\] The polyphenols yield was achieved to 2.135 mg/g for red-flesh apple of XINJIANG under the optimized conditions. This result can provide a reference for the actual production of polyphenols.