In order to evaluate the content of polyphenol and flavonoid, as well as antioxidant activity in different parts of Akebia trifoliata., the ultrasonic-assisted and reflux method were used. The content of polyphenol and flavonoids, and antioxidant capacity in different parts were compared. The results showed that the ultrasonic extraction method was more efficient than the reflux extraction method, among the all extracts, the highest content of polyphenol and flavonoid, and strongest antioxidant capacity was found in cane extract by ultrasonic-assited (CSJ). The contents of the total polyphenol, flavonoid in CSJ as (63.033±0.482) mg/g, (26.395±0.731) mg/g, respectively, and the DPPH, ABTS, FRAP antioxidant activity value in CSJ as (380.860±5.462) μmol Trolox/g, (337.181±1.586) μmol Trolox/g and (387.406±1.773) μmol Trolox/g, respectively. The polyphenol, flavonoids content and antioxidant activity for all extracts were showing the following tendency: cane ＞ leaf ＞ peel ＞ seed ＞ sarcocarp. Spearman correlation study shows that the content of polyphenol, flavonoids and strongly related to the antioxidant activity. The research results provide experimental basis for deep processing of Akebia Trifoliata..