Aflatoxin B1-contaminated corn (ACC) (83.0 μg/kg) was treated for 40 min at 90 mg/L ozone. And mice were used to assess the vivo toxicity of ozone treated ACC. Results indicated that compared with basal feedstuff, the mean weight of mice fed with ACC significantly decreased (P＜0.05). Whereas, the liver and kidney/body weight ratio increased significantly (P＜0.05). Alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in serum increased significantly (P＜0.05). The total protein (TP), albumin (ALB), and globulin (GLB) content significantly decreased (P＜0.05). When mice were fed with the ozone-treated ACC, no significant differences were observed in the mice mean weight, as well as the liver and kidney/body weight ratio. Additionally, no significant differences occurred in the major serum indexes ALT, TP, ALB, and GLB (P＞0.05). Meanwhile, AST and ALP increased significantly (P＜0.05). The study indicated that ACC can lead to significant changes in various physiological characteristics and biochemical indexes in liver and kidney tissues, while the ozone-treated ACC could significantly improve these changes.