By using Micrococcus luteus and Pseudomonas aeruginosa as indicator bacteria, the antibacterial properties for metabolites of Lactobacillus plantarum SJ35 was determined by Oxford cup double agar diffusion method, and the fermentation conditions of strain were selected by single factor experiment. The antibacterial effect of compound preservative was determined which was prepared by the combination of sodium chlorite and metabolites. Results indicated that metabolites of Lactobacillus plantarum SJ35 had good antibacterial activity; it was stable in high temperature, acid conditions, and sensitive to proteases; it also had a wide antibacterial spectrum. The optimal fermentation conditions were as followed: fermentation temperature 34 ℃, fermentation time 36 h and initial pH 6.0. The antibacterial circle diameter of metabolites was 25.68 mm and 19.96 mm for Micrococcus luteus and Pseudomonas aeruginosa respectively. The compound preservative which was prepared by the combination of 80% SJ35 metabolites and 600 mg/L sodium chlorite, could not only enhance inhibition of bacteria but also be antifungal.