Neferine was studied as object in this study. Using total reducing power, hydroxyl radical system, superoxide radical system, DPPH system, and schaal oven-storage test to study the antioxidant activities and antioxidant activity in rapeseed oil of neferine from lotus powder, which prepared by HSCCC. The results showed: compared with V_C, neferine showed better total reducing power and scavenging effect in free radical system, which its maximum scavenging rate of hydroxyl radical,superoxide radical and DPPH radical was 62.79%, 76.23%, 93.57%, respectively. In addition, different neferine dosage had both dose-effect on the relationship with the antioxidant effect of rapeseed oil. The study showed that 0.1% neferine had better effect than 0.02% TBHQ and 0.05% V_E, and 0.05% V_E got the worst effect.Moreover, combination of V_C, citric acid with neferine had a certain synergy effect to rapeseed oil, and citric acid’s complex effect was the best, V_C followed.