Effects of sodium citrate were investigated on the growth, the serum biochemical parameters and intestinal flora of mice. Mice were randomly divided into four groups: control group, low-dose group (added 0.65% sodium citrate), middle-dose group (added 2.6% sodium citrate) and high-dose group (added 5.2% sodium citrate).The diet intervention lasted for four weeks. Stool samples and weight data were collected weekly, and the composition of intestinal microbiota and the serum biochemical parameters were determined at the end of the fourth week. Results showed that the addition of sodium citrate made the body weight of mice less than control group. And the high-dose group’s high density lipoprotein cholesterol (HDL-C) had a significantly increased. The beta diversity and the ratio of Fimicutes to Bacteriodetes were changed by intake of sodium citrate. At the genus level, Corynebacterium and Staphylococcus were detected in the high-dose group. Results indicated that the intestinal flora of mice could be affected by intake of high dose of sodium citrate.