A method was developed for determination of glyphosate and aminomethyl phosphonic acid residues in tea by QuEChERS and UPLC/MS/MS. The samples were extracted with water and cleaned up with dispersive-solid phase extraction. The target analytes were separated by CORTECSTM UPLC C₁₈₊ column with gradient elution using acetonitrile and 0.1% ammonium hydroxide solution as mobile phases. Negative electrospray ionization with multiple reaction monitoring mode was used in the detection. Internal standard method was used to determine the results. The linear ranges were 5 to 500 μg/L. The limit of detection (LOD) of this method were 10 μg/kg, and the limit of quantitation (LOQ) were 30 μg/kg. The recoveries of glyphosate and aminomethyl phosphonic acid were 90.5%～93.5% and 99.2%～102.4% respectively, with relative the standard deviations of 4.1%～7.2% and 3.4%～8.3% at three spiked concentration levels of 30 μg/kg, 100 μg/kg and 500 μg/kg. The simplicity, sensitivity and good precision of the method made it be well suitable for determination of glyphosate and aminomethyl phosphonic acid residues in tea.