The Partial Least Square Regression (PLSR) analysis was used to study the specific influence of protein and starch contents on sensory quality of 10 different kinds of commercial cigarettes. The results showed that there were significant negative correlation between protein, starch and astringency, throat irritation, nasal irritation, indicating that the lower protein and starch content in tobacco, the lower astringency, throat irritation, nasal irritation and higher sensory quality.