Objective To broaden the linear range of ELISA for deoxynivalenol detection.Methods This paper obtains three antibodies with different sensitivities via mouse immunization, cell fusion and screening, ascites preparation and antibody purification. By optimizing the working concentration and composition ratio of the antibody synergistic combination, the optimal antibody synergistic combination ratio is applied to ELISA to determine the linear range.Results The optimal reaction conditions for the ic-ELISA based on synergistic antibodies are as follows: the optimal working concentrations of the coating antigen and antibody are 0.3 and 0.1 μg/mL respectively. The linear range of the synergistic antibody is obviously broadened compared with the initial single antibody. When 1E2-mAb and 2A11-mAb are mixed at a volume ratio of 4:1, the linear range of the synergistic antibody is 47.7 times higher than that of the single antibody 1E2-mAb and 148.6 times higher than that of the single antibody 2A11-mAb.Conclusion This antibody synergistic combination strategy can effectively broaden the linear range of ELISA.