Objective To investigate the effects of wrinkled pea meal replacement powder on glucose and lipid metabolism disorders and gut microbiota.Methods A 12-week intervention experiment was conducted using a high-fat diet-fed mouse model. Changes in key indicators, including mouse phenotypes, blood biochemical parameters, histopathology, and gut microbial composition, were systematically monitored.Results Wrinkled pea meal replacement powder significantly reduced fasting blood glucose, area under the blood glucose curve, and serum levels of total cholesterol, triglycerides, and low-density lipoprotein cholesterol in high-fat-fed mice (P<0.05). It effectively alleviated hepatic steatosis, reduced serum alanine aminotransferase levels (P<0.05), and significantly mitigated hepatocyte damage. Additionally, consumption of wrinkled pea meal replacement powder significantly regulated gut microecological imbalance induced by a high-fat diet, increased gut microbial richness, decreased the Firmicutes/Bacteroidetes ratio, and increased the relative abundance of beneficial bacteria, including norank_f_Muribaculaceae, norank_o_Clostridia_UCG-014, and Bifidobacterium.Conclusion Wrinkled pea meal replacement powder has potential application value in improving glucose and lipid metabolism and regulating gut health.