固相萃取—液相色谱串联质谱法测定鸡蛋中8种杀菌剂残留量
CSTR:
作者:
作者单位:

1广电计量检测(湖南)有限公司,湖南 长沙 410221;2中国检验认证集团湖南有限公司,湖南 长沙 410007

作者简介:

通讯作者:

李青(1987—),男,中国检验认证集团湖南有限公司高级工程师,硕士。E-mail:287476045@qq.com

中图分类号:

基金项目:

湖南省自然科学基金项目(编号:2024JJ8259)


Determination of eight fungicide residues in eggs by solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry
Author:
Affiliation:

1GRG Metrology & TEST (Hunan) Co., Ltd., Changsha, Hunan 410221, China;2China Certification & Inspection Group Hunan Co., Ltd., Changsha, Hunan 410007, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 建立一种固相萃取结合液相色谱串联质谱测定鸡蛋中8种杀菌剂(唑嘧菌胺、三唑酮、三唑醇、粉唑醇、双炔酰菌胺、吡噻菌胺、吡唑萘菌胺、咪鲜胺)残留的分析方法。方法 样品经乙腈超声提取,提取液过ProElut DPC固相萃取柱净化,净化液减压浓缩后用乙腈定容,采用Kinetex C18色谱柱(2.6 μm,2.1 mm×100 mm),以0.1%甲酸水—乙腈为流动相梯度洗脱,电喷雾电离,正离子模式下多反应检测,外标法定量。结果 8种杀菌剂在一定浓度范围内线性关系良好(R2>0.99),方法检出限为0.03~0.50 μg/kg,定量限为0.10~2.00 μg/kg,平均回收率为81.6%~107.0%。结论 该方法操作简便、准确度高、再现性好,适用于鸡蛋中杀菌剂残留量的检测。

    Abstract:

    Objective To establish an analytical method for the determination of residues of eight fungicides (ametoctradin, triadimefon, triadimenol, flutriafol, mandipropamid, penthiopyrad, isopyrazam, and prochloraz) in eggs by solid-phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS).Methods The samples are ultrasonically extracted with acetonitrile. The extracts are purified using ProElut DPC solid-phase extraction cartridges. The purified eluate is concentrated under reduced pressure and reconstituted with acetonitrile. Chromatographic separation is performed on a Kinetex C18 column (2.6 μm, 2.1 mm×100 mm) with gradient elution using 0.1% formic acid in water and acetonitrile as the mobile phase. Detection is carried out using electrospray ionization (ESI) in positive ion mode with multiple reaction monitoring (MRM), and quantification is performed using the external standard method.Results The eight fungicides exhibit good linearity within their respective concentration ranges (R2>0.99). The limits of detection (LODs) range from 0.03 to 0.50 μg/kg, and the limits of quantification (LOQs) range from 0.10 to 2.00 μg/kg. The average recoveries range from 81.6% to 107.0%.Conclusion The established method is simple, accurate, and reproducible, making it suitable for the determination of fungicide residues in eggs.

    参考文献
    相似文献
    引证文献
引用本文

梁军,陈雄,王宇,等.固相萃取—液相色谱串联质谱法测定鸡蛋中8种杀菌剂残留量[J].食品与机械,2026,42(5):77-83.
LIANG Jun, CHEN Xiong, WANG Yu, et al. Determination of eight fungicide residues in eggs by solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry[J]. Food & Machinery,2026,42(5):77-83.

复制
分享
相关视频

文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2026-02-25
  • 最后修改日期:2026-05-06
  • 录用日期:
  • 在线发布日期: 2026-06-17
  • 出版日期:
文章二维码
×
《食品与机械》
友情提示
友情提示 一、 近日有不少作者反应我刊官网无法打开,是因为我刊网站正在升级,旧网站仍在百度搜索排名前列。请认准《食品与机械》唯一官方网址:http://www.ifoodmm.com/spyjx/home 唯一官方邮箱:foodmm@ifoodmm.com; 联系电话:0731-85258200,希望广大读者和作者仔细甄别。