Mycotoxins are secondary metabolites produced by fungi during their growth. Widely observed in grains and feeds, these toxins pose a significant threat to both human and animal health, resulting in substantial economic losses. Therefore, this paper systematically reviews the principles, application effects, advantages, and disadvantages of mainstream detoxification and degradation technologies, including physical detoxification methods (heat treatment, radiation, adsorption, low-temperature plasma, and photocatalysis), chemical detoxification methods (ammonia treatment, fumigation, and ozonation), as well as biodegradation methods (microbial adsorption, microbial degradation, and enzymatic degradation). It specifically introduces the breakthroughs of new technologies such as low-temperature plasma, novel nano-adsorbent materials, and high-temperature resistant detoxifying enzymes. Additionally, it elaborates on the innovative applications and existing challenges of artificial intelligence technology in the field of mycotoxin detoxification. Future research should focus on multi-technology integration and intelligent upgrading, while developing efficient, safe, and economical comprehensive detoxification and degradation strategies to provide scientific support for grain and food safety.