Objective To investigate the air flow organization and temperature distribution inside a large fruit and vegetable elevated warehouse and analyze the formation mechanism.Methods Experimental measurement and CFD numerical simulation are performed to analyze the actual air distribution inside a large fruit and vegetable elevated warehouse.Results The average temperature of the horizontal plane in the loading area decreases with the decrease in height. The maximum average temperatures of the upper (H=12.3 m), middle (H=7.3 m), and lower (H=2.3 m) layers are 3.05 ℃, 2.86 ℃, and 2.31 ℃, respectively. The temperature differences between the upper and middle layers and between the lower and middle layers are 0.19 ℃ and 0.55 ℃, respectively. The warehouse employs a roof-mounted air cooler combined with a fabric air duct system, with uniform air flow organization. Under the influences of the resistance of the container and the air supply and suction of the fabric air duct, the surface temperature difference is larger for the cargo closer to the middle of the warehouse, and a high-temperature zone is formed in the middle and upper part of the middle aisle (the return air area of the chiller).Conclusion The fabric air duct design achieves uniform air flow organization in the warehouse, while the cooling effect in some areas is not satisfactory due to the dense containers and respiratory heat accumulation, and the non-linearity of temperature rise requires the optimization of insulation layer to save energy and improve equipment life.