Objective To establish a new Sin-QuEChERS nano-triple quadrupole liquid chromatography-mass spectrometry (UPLC-MS/MS) method for detecting emamectin benzoate residues in rice.Methods After extraction with 0.1% formic acid-acetonitrile, the sample is purified with N-primary secondary amine (PSA) and multi-walled carbon nanotube (MWCNTs) fillers. The mobile phase is a gradient elution of 0.1% formic acid water and acetonitrile. Separation is performed using a C₁₈ chromatographic column, and the detection is carried out using a triple quadrupole liquid chromatography-tandem mass spectrometry system.Results The quantification limit of emamectin benzoate is 0.001 5 mg/kg, and the detection limit of the method is 0.000 5 mg/kg. A good linear relationship is observed for emamectin benzoate in the concentration range of 1~100 ng/mL, with a correlation coefficient greater than 0.999 5. At 2, 10, and 50 μg/kg spiking levels, the average recovery rate of emamectin benzoate is 86.9%~110.2%, with a relative standard deviation of 0.98%~7.21%.Conclusion This method is efficient, simple, stable, and highly sensitive, making it suitable for detecting emamectin benzoate in rice.