双翅六道木嫩叶果胶超声波辅助酶法提取及结构表征
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1.湖南农业大学食品科技学院,湖南 长沙 410128;2.长沙市农产品质量监测中心,湖南 长沙 410003

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周辉(1980—),男,湖南农业大学教授,博士。E-mail:paradise917@163.com

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湖南省重点研发项目(编号:2023NK2040)


Ultrasound-assisted enzymatic extraction and structural characterization of pectin from the tender leaves of Abelia macrotera
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1.School of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China;2.Agricultural Product Quality Monitoring Center of Changsha, Changsha, Hunan 410003, China

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    摘要:

    目的 明确双翅六道木嫩叶中果胶的理化性质、微观结构、单糖组成、相对分子质量及结构特征。方法 采用超声波辅助纤维素酶法提取双翅六道木嫩叶果胶,扫描电镜下观察其微观结构,利用高效液相色谱法测定其单糖组成,通过多糖水解衍生化测定其相对分子质量,并利用红外光谱法推测其可能的结构。结果 双翅六道木嫩叶果胶得率为23.38%,其酯化度为1.17%、半乳糖醛酸含量为72.16%,该果胶呈片状且含有裂痕,其单糖主要由半乳糖醛酸、葡萄糖、阿拉伯糖、半乳糖、鼠李糖、甘露糖和木糖等组成,重均分子量(Mw)和数均分子量(Mn)分别为7.069×105,3.434×105,Mw/Mn为2.06,并具有典型的果胶吸收光谱特征。结论 超声波辅助酶法提取的双翅六道木嫩叶果胶是一种结构线性度较低,以含有较短侧链的RG-I型结构为主的符合商业要求的果胶。

    Abstract:

    Objective To clarify the physicochemical properties, microscopic structure, monosaccharide composition, relative molecular mass, and structural characteristics of pectin in the tender leaves of Abelia macrotera.Methods Pectin from the tender leaves of Abelia macrotera was extracted by ultrasound-assisted cellulase method. The microscopic structure was observed under scanning electron microscope. The monosaccharide composition was determined by high performance liquid chromatography. The relative molecular mass was determined by polysaccharide hydrolysis derivatization. The possible structure was inferred by infrared spectroscopy.Results The yield of pectin from the tender leaves of Abelia macrotera is 23.38%, the esterification degree was 1.17%, and the galacturonic acid content was 72.16%. The pectin is in the form of flakes with cracks. The monosaccharides mainly consist of galacturonic acid, glucose, arabinose, galactose, rhamnose, mannose and xylose. The weight average molecular weight (Mw) and number average molecular weight (Mn) are 7.069×105 and 3.434×105, respectively, with an Mw/Mn of 2.06. It has typical spectrum characteristics of pectin absorption.Conclusion The pectin extracted from the tender leaves of Abelia macrotera by ultrasound-assisted enzyme method has a low linear structure and is mainly composed of RG-I type structure containing shorter side chains, which meets the commercial requirements.

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毛琪琪,罗扬,王姜林,等.双翅六道木嫩叶果胶超声波辅助酶法提取及结构表征[J].食品与机械,2025,41(7):18-23.
MAO Qiqi, LUO Yang, WANG Jianglin, et al. Ultrasound-assisted enzymatic extraction and structural characterization of pectin from the tender leaves of Abelia macrotera[J]. Food & Machinery,2025,41(7):18-23.

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  • 收稿日期:2024-11-18
  • 最后修改日期:2025-04-11
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  • 在线发布日期: 2025-07-12
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