Objective To explore the biosynthesis method of naringin, a bioactive compound in citrus.Methods Engineered Saccharomyces cerevisiae strains for naringenin biosynthesis are constructed through enzyme screening, multi-copy integration, and precursor supply. Subsequently, de novo biosynthesis of naringin from D-xylose is achieved by introducing the naringin biosynthetic pathway and optimizing UDP-glucose supply.Results The optimal flavonoid synthase is identified as PhCHS by screening the key enzymes of the naringenin pathway. The naringenin biosynthesis pathway is further integrated at multi-copy sites. The constructed strain Z39M2 can synthesize 36.53 mg/L naringenin. The overexpression of CAT2, xPK-PTA pathway, and ACC1m optimizes malonyl-CoA supply and constructs strain Z44, with a naringenin production increasing to 72.96 mg/L. Finally, the naringin biosynthesis pathway introduction enables the successful biosynthesis of 35.60 mg/L naringin. Further overexpression of PGM1 and UGP1 elevates naringin production to 76.67 mg/L.Conclusion An engineered S. cerevisiae strain capable of de novo biosynthesis of naringin from D-xylose is successfully constructed by metabolic engineering.