Quinolones (QNs) are highly favored for their broad-spectrum antibacterial effects. However, the overuse of QNs in animal feed can lead to their residues entering the food chain, thus posing potential hazards to the environment and human health. This paper summarizes the main immunoassay techniques for quinolone residues, analyzes their advantages and application prospects, and discusses the challenges and future development directions of immunoassay techniques.