Objective To reduce the phosphorus content in Korean pine seed oil and develop an efficient degumming method.Methods An ultrasound-assisted method was used, and the degumming process of Korean pine seed oil was optimized through orthogonal experiments. The fatty acid composition, content, and antioxidant capacity of the degummed oil were analyzed.Results The optimal degumming conditions for Korean pine seed oil were ultrasonic power of 420 W, degumming temperature of 50 ℃, degumming time of 3.5 h, enzyme dosage of 40 mg/kg, and pH 4.8. Under these conditions, the phosphorus content of the degummed oil was (5.18 ± 0.24) mg/kg, and the proportion of pinolenic acid in total fatty acids was 15.46%. When the sample concentration was 40 mg/mL, the DPPH and ABTS⁺ radical scavenging rates were (91.42 ± 0.38)% and (92.47 ± 0.84)%, respectively.Conclusion The optimized ultrasound-assisted enzymatic degumming process for Korean pine seed oil yields high-quality degummed oil with good antioxidant activity.