Objective To develop a rapid and accurate method for the determination of carbendazim residues in vegetable oils.Methods Three-dimensional fluorescence spectroscopy combined with chemometrics was used for the quantitative analysis of carbendazim residues in vegetable oils. Three different vegetable oil extracts were added to carbendazim to prepare samples, and their three-dimensional fluorescence spectra were measured. Parallel factor (PARAFAC), full-rank parallel factor (FPA-PARAFAC), and alternating trilinear decomposition (ATLD) were used to analyze carbendazim in the samples. The figures of merit (FOM), such as selectivity, sensitivity, detection limit, and quantification limit, were calculated for the three algorithms, and tested using the elliptical joint confidence region (EJCR).Results The recovery rates of carbendazim for PARAFAC, FPA-PARAFAC, and ATLD were 96.98%~112.64%, 97.05%~112.36%, and 93.07%~104.47%, respectively, with the average recovery rate for ATLD being closer to 100%. In FOM and EJCR analysis, ATLD outperformed the other two algorithms.Conclusion The use of three-dimensional fluorescence spectroscopy combined with chemometrics for the determination of carbendazim residues in vegetable oils provides excellent quantitative results.