Objective To establish a rapid detecting method for the determination of dimethyl fumarate in beverages by ultrasound-assisted dispersive liquid-liquid micro-extraction coupled with high performance liquid chromatography.Methods The beverage samples were spiked with extractant and dispersant, extracted by ultrasonic extraction, centrifuged and determined by high performance liquid chromatography.Results The optimal microextraction conditions were 100 μL of chloroform as extractant, 300 μL of acetone as dispersant, and a sonication time of 4 min. Under the optimal extraction conditions, dimethyl fumarate had a linear range of 0.1~100.0 μg/mL with a detection limit of 0.02 μg/L. The relative standard deviation (RSD) of the method was less than 3.7%, and the spike recovery rate was between 75.0% and 85.0%.Conclusion The method is simple, rapid, reproducible, sensitive and environmentally friendly, and is suitable for the qualitative and quantitative analysis of dimethyl fumarate in beverages.