竹叶花椒挥发油化学成分及其抗氧化活性研究
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(1. 四川中医药高等专科学校绵阳市中药资源开发利用重点实验室 ,四川 绵阳 621010; 2. 四川中医药高等专科学校川西北中药材资源研究与开发利用实验室 ,四川 绵阳 621010; 3. 四川中医药高等专科学校药学院 ,四川 绵阳 621010)

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通讯作者:

梁晓峰(1975—),男,四川中医药高等专科学校教授,博士。E-mail:454192439@qq.com

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四川省中医药管理局科学技术研究专项课题(编号:2023MS334);绵阳市中药资源开发利用实验室开放基金课题(编号:24ZYKF03)


Study on chemical components of the essential oil from Zanthoxylum armatum DC and its antioxidant activity
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(1. Mianyang Key Laboratory for the Development and Utilization of Traditional Chinese Medicine Resources , Sichuan College of Traditional Chinese Medicine , Mianyang , Sichuan 621010 , China; 2. Laboratory of Research Development and Utilization on Traditional Chinese Medicine Resources in Northwest Sichuan , Sichuan College of Traditional Chinese Medicine , Mianyang , Sichuan 621010 , China; 3. College of Pharmacy of Sichuan College of Traditional Chinese Medicine , Mianyang , Sichuan 621010 , China)

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    摘要:

    [目的]研究竹叶花椒挥发油成分、抗氧化活性和抗氧化稳定性。[方法]采用超临界 CO2萃取法(SFE-CO2)和水蒸气蒸馏法 (HD)提取挥发油,利用气相色谱—质谱联用 (GC-MS)法分析其化学成分;采用 DPPH自由基、ABTS自由基清除法评价竹叶花椒挥发油的抗氧化活性,并研究 pH、温度、防腐剂和甜味剂等对竹叶花椒挥发油抗氧化活性的影响。[结果]GC-MS分析显示,竹叶花椒挥发油主要含有醇类、烯类物质;两种方法得到的挥发油均具有较强的抗氧化活性,SFE-CO2和HD所得挥发油对 ABTS自由基的半抑制质量浓度分别为 0.248,0.580 mg/mL,对DPPH自由基的半抑制质量浓度分别为 4.620,8.920 mg/mL;pH、温度、防腐剂和甜味剂能够影响挥发油的抗氧化活性。[结论]两种提取方法所得挥发油的化学成分存在差异,但均具有较强的抗氧化活性,且在一定质量浓度范围内稳定,可作为天然抗氧化剂的潜在来源。

    Abstract:

    [Objective] This study aimed to investigate the composition,antioxidant properties,and stability of the essential oil extracts from Zanthoxylum armatum DC.[Methods] Essential oil was extracted using supercritical CO2 extraction (SFE-CO2) and steam distillation (HD).Gas chromatography -mass spectrometry (GC-MS) was employed to analyze the chemical composition of the oils.Antioxidant activity was evaluated using DPPH and ABTS free radical scavenging assays.Additionally,the effects of pH,temperature,preservatives,and sweeteners on the antioxidant activity of the essential oil were assessed.[Results]] GC-MS analysis revealed that alcohols and olefins were the primary components of the essential oils.Both extraction methods yielded oils with strong antioxidant properties.The half -maximal inhibition concentration (IC50) for ABTS radicals was 0.248 mg/mL for SFE -CO2 and 0.580 mg/mL for HD,while for DPPH radicals,the IC50 values were 4.620 mg/mL and 8.920 mg/mL.Variations in pH values,temperature,preservatives and sweeteners influenced the antioxidant activity of the oils.[Conclusion] The chemical composition of essential oils differs based on the extraction method,but both demonstrate strong antioxidant activity and stability under certain conditions.These findings suggest that Z.armatum essential oils could serve as a promising natural source of antioxidants.

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周孟焦,陈 凯,孙李娜,等.竹叶花椒挥发油化学成分及其抗氧化活性研究[J].食品与机械,2024,40(12):25-31.
ZHOU Mengjiao, CHEN Kai, SUN Lina, et al. Study on chemical components of the essential oil from Zanthoxylum armatum DC and its antioxidant activity[J]. Food & Machinery,2024,40(12):25-31.

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  • 收稿日期:2024-02-28
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  • 在线发布日期: 2025-02-18
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