绿茶中主要儿茶素、氨基酸单体呈味分属性二次多项回归模型建立
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(1. 贵州农业职业学院 ,贵州 贵阳 551400; 2. 贵州省茶叶研究所 ,贵州 贵阳 550006; 3. 铜仁职业技术学院 ,贵州 铜仁 554309)

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潘科(1984—),男,贵州省农业科学院茶叶研究所研究员,博士。E-mail:148450502@qq.com

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贵州省科技支撑项目(编号:黔农科科技创新[2023]12号,GZCYCYJSTX-05)


A quadratic multinomial regression model was established for taste properties of main catechins and amino acid monomers in green tea
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(1. Guizhou Vocational College of Agriculture , Guiyang , Guizhou 551400 , China; 2. Guizhou Tea Research Institute , Guiyang , Guizhou 550006 , China; 3. Tongren Vocational and Technical College , Tongren , Guizhou 554309 , China)

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    摘要:

    [目的]探究绿茶主要滋味单体混合后的呈味强度。[方法]采用电子舌检测技术,以绿茶中主要的 3种儿茶素 [表没食子儿茶素没食子酸酯 (EGCG)、没食子儿茶素没食子酸酯 (GCG)、表儿茶素没食子酸酯 (ECG)]和3种氨基酸单体(天冬氨酸、谷氨酸、茶氨酸)为研究对象,结合二次多项式回归分析方法构建绿茶滋味属性评价模型。[结果]所配比溶液中智能感官强度遵循鲜味 >涩味 >苦味。ECG、EGCG与天冬氨酸和谷氨酸的互作与苦味具有正效应;天冬氨酸、谷氨酸分别与茶氨酸的互作对苦味强度有负效应;GCG、ECG对涩味有正效应,儿茶素与氨基酸间互作和谷氨酸分别与天冬氨酸、茶氨酸互作对涩味有负效应;谷氨酸、茶氨酸、儿茶素与氨基酸间互作及儿茶素间互作对鲜味有正效应,ECG、茶氨酸、儿茶素与氨基酸的互作、天冬氨酸与茶氨酸的互作、谷氨酸与茶氨酸的互作对鲜味有负效应。构建单体滋味属性评价模型为 Y(苦)=17.033 1+0.927 0X4+0.002 5X23+0.000 2X26+0.001 9X2X5+0.009 3X4X5-0.003 7X4X6-0.000 7X5X6;Y(涩)=-32.097 5+0.199 8X2+0.455 3X3+1.222 9X4+0.000 6X22+0.004 4X25-0.000 1X1X6-0.002 6X2X3-0.008 1X3X4-0.017 3X4X5+0.001 4X5X6;Y(鲜)=80.221 8-0.703 7X3+0.555 6X5-0.355 4X6+0.000 7X26-0.001 6X1X2-0.002 7X1X5+0.000 8X1X6+0.007 9X2X3-0.009 7X2X5+0.014 3X3X5-0.002 4X4X6-0.004 8X5X6;模型相关系数均 >0.890 1,决定系数均 >0.792 2,参考模型滋味强度鲜味为 15.17~53.58,苦味为 3.03~15.76,涩味为 5.94~16.52。[结论]以茶氨酸、谷氨酸、天冬氨酸、EGCG、GCG和ECG单体组成的溶液,一定程度上能模拟绿茶茶汤滋味强度,根据 3个模型能匹配出绿茶滋味属性的最高强度和最低强度。

    Abstract:

    [[Objective ]] To study the flavor intensity of green tea after mixing the main flavor monomers.[[Methods ]] Three main catechins [epigallocatechin gallate (EGCG ),epigallocatechin gallate (GCG ),epicatechin gallate (ECG )] and three amino acid monomers (aspartic acid,glutamic acid and theanine ) in green tea were studied by electronic tongue detection technique.The evaluation model of green tea taste attribute was constructed by quadratic polynomial regression analysis.[[Results]] The intelligent sensory intensity in the matching solution followed the order of umami > astringency > bitter taste.ECG,EGCG interaction with aspartate and glutamic acid had positive effect on bitter taste.Aspartate and glutamic acid had negative effects on the bitterness intensity of theanine.GCG and ECG had a positive effect on astringency,the interaction between catechin and amino acid and glutamic acid with aspartic acid and theanine had a negative effect on astringency.The interaction between glutamic acid,theanine,catechin and amino acid and the interaction between catechin and catechin had |a positive effect on umami taste,while the interaction between ECG,theanine,catechin and amino acid,aspartic acid and theanine,glutamic acid and theanine had a negative effect on umami taste.The evaluation model of monomer taste attribute was Y (bitter )=17.033 1+0.927 0X4+0.002 5X23+0.000 2X26+0.001 9X2X5+0.009 3X4X5-0.003 7X4X6-0.000 7X5X6.Y=-32.097 5+0.199 8X2+0.455 3X3+1.222 9X4+0.000 6X22+0.004 4X25-0.000 1X1X6-0.002 6X2X3-0.008 1X3X4-0.017 3X4X5+0.001 4X5X6;Y (fresh )=80.221 8-0.703 7X3+0.555 6X5-0.355 4X6+0.000 7X26-0.001 6X1X2-0.002 7X1X5+0.000 8X1X6+0.007 9X2X3-0.009 7X2X5+0.014 3X3X5-0.002 4X4X6-0.004 8X5X6;The correlation coefficients of the model were all > 0.890 1,and the determination coefficients were all >0.792 2.The reference model taste intensity was 15.17~53.58 in umami,3.03~15.76 in bitterness,and 5.94~16.52 in astringency.[[Conclusion ]] The solution composed of theanine,glutamic acid,aspartic acid,EGCG,GCG and ECG monomers can simulate the flavor intensity of green tea soup to a certain extent,and the highest and lowest intensity of green tea taste properties can be matched according to the three models.

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冉乾松,刘忠英,刘 锡,等.绿茶中主要儿茶素、氨基酸单体呈味分属性二次多项回归模型建立[J].食品与机械,2024,40(10):14-20.
RAN Qiansong, LIU Zhongying, LIU Xi, et al. A quadratic multinomial regression model was established for taste properties of main catechins and amino acid monomers in green tea[J]. Food & Machinery,2024,40(10):14-20.

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  • 收稿日期:2023-10-21
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  • 在线发布日期: 2025-02-18
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