超声辅助离子液体提取猪苓多糖的工艺优化及抗氧化性研究
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(1. 陕西理工大学化学与环境科学学院 ,陕西 汉中 723000 ; 2. 陕西理工大学陕西省催化基础与应用重点实验室 ,陕西 汉中 723000)

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李志洲(1969—),男,陕西理工大学教授,硕士。E-mail:729838161@qq.com

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陕西省教育厅项目(编号:23JK0361)


Study on the optimization of the process of extracting Polyporus umbellatus polysaccharides by ultrasonic-assisted ionic liquid and its antioxidant activity
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(1. Shaanxi University of Technology , College of Chemistry and Environmental Sciences , Hanzhong , Shaanxi 723000 , China ; 2. Shaanxi Institute of Technology Shaanxi Province Catalytic Basic and Application Key Laboratory , Hanzhong , Shaanxi 723000 , China)

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    摘要:

    [目的]以陕南猪苓菌为原料,开发高效提取猪苓多糖的工艺,并对提取物的抗氧化活性进行考察。[方法]采用超声辅助离子液体法提取猪苓多糖,在单因素试验的基础上进行五因素四水平的正交试验,考察提取时间、提取温度、料液比及 1-乙基 -3-甲基咪唑醋酸盐 ([EMIM ]AC)质量浓度对猪苓多糖提取率的影响。对超声辅助离子液体提取猪苓菌中的猪苓多糖的工艺进行优化。同时通过 DPPH 自由基清除和羟自由基清除探究猪苓多糖的抗氧化能力。[结果]超声辅助离子液体提取猪苓多糖的最佳工艺条件为超声时间 60 min,提取温度 70 ℃,料液比 1∶25 (g/mL ),[EMIM ]AC质量浓度 3 g/L,在此条件下猪苓多糖提取率为 8.74%。猪苓多糖对 DPPH 自由基、羟自由基均具有良好的清除作用,并呈剂量依赖性。[结论]超声辅助离子液体能有效提高猪苓多糖提取率,且提取物具有较强的抗氧化活性。

    Abstract:

    [[Objective ]] This study aimed to develop an efficient extraction process for polysaccharides from southern Shaanxi Polyporus umbellatus using ultrasonic -assisted ionic liquid extraction and evaluate the antioxidant activity of the extract. [Methods ] Use the ultrasonic auxiliary ionic liquid method to extract poronalt polysaccharides,and conduct five -level orthogonal experiments on the basis of single -factor testing.Examine the effects of extracting time,extraction temperature,material liquid ratio and 1-ethyl -3-methylimramidazole acetate quality concentration of the extraction rate of pomollagium polysaccharides.Optimize the process of extracting the poriaon polysaccharide in the liquid liquid of the ultrasonic auxiliary ion liquid.Additionally,the DPPH free radicals clearance and OH free radicals to remove the antioxidant capacity of poronaldium polysaccharides.[Results ] Optimal extraction conditions were achieved with a 60 min ultrasonic time,extraction temperature of 70 ℃,a solid -to-liquid ratio of 1:25 (g/mL ),and a [EMIM ]AC was 3 g/L.Under this condition,the extraction rate of polymal polysaccharides is 8.74%.The extracted polysaccharides showed significant antioxidant activity,effectively scavenging DPPH and OH free radicals.[ConclusionConclusion ] The Ultrasonic -Assisted Ionic Liquid extraction process is efficient and feasible for extracting P.umbellatus polysaccharides,yielding a high extraction rate and strong antioxidant activity.This method enhances the potential application of P.umbellatus polysaccharides as natural antioxidants.

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祝 苗,李志洲,刘军海,等.超声辅助离子液体提取猪苓多糖的工艺优化及抗氧化性研究[J].食品与机械,2024,40(9):167-173.
ZHU Miao, LI Zhizhou, LIU Junhai, et al. Study on the optimization of the process of extracting Polyporus umbellatus polysaccharides by ultrasonic-assisted ionic liquid and its antioxidant activity[J]. Food & Machinery,2024,40(9):167-173.

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  • 收稿日期:2024-04-07
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  • 在线发布日期: 2025-02-18
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