柑橘皮油胞层提取物的抗氧化活性与抑菌活性
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(1. 湖南农业大学食品科学技术学院 ,湖南 长沙 410128 ; 2. 鹿儿岛大学农学部 ,日本 鹿儿岛 8900065 ; 3. 湖南省辣椒精深加工工程技术研究中心 ,湖南 长沙 410128)

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李清明(1973—),男,湖南农业大学教授,博士。E-mail:lqm@hunau.edu.cn

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Study on antioxidant and antibacterial activity of extracts from flavedo of citrus peel
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(1. College of Food Science and Technology , Hunan Agricultural University , Changsha , Hunan 410128 , China ; 2. Faculty of Agriculture , Kagoshima University , Kagoshima 8900065 , Japan ; 3. Engineering Research Center for Pepper Deep Processing of Hunan Province , Changsha , Hunan 410128 , China)

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    摘要:

    [目的]提高柑橘皮的深加工和高值化利用。[方法]利用不同溶剂提取酢橘、代代酸橙和河内晚柑果皮油胞层活性成分,测定提取物的 DPPH 自由基和 ABTS 自由基清除率,并通过抑菌圈、微量肉汤稀释法和抑菌动力学 3个指标综合评价柑橘皮油胞层提取物的抑菌活性。[结果]甲醇提取物的得率、活性物质含量、抗氧化活性和抑菌活性均优于石油醚提取物和正己烷提取物。提取物的抗氧化活性大小为酢橘 >代代酸橙 >河内晚柑,酢橘皮油胞层甲醇提取物的 DPPH 自由基和ABTS 自由基清除率分别为 83.86%,69.14%。各提取物均能有效抑制 4种食源性致病细菌生长,其中对金黄色葡萄球菌的抑制作用最显著 (P<0.05);3种柑橘皮油胞层的抑菌活性大小为酢橘 >代代酸橙 >河内晚柑,酢橘皮油胞层甲醇提取物对大肠杆菌、沙门氏菌和金黄色葡萄球菌的抑菌圈为 10~15 mm,属于中度敏感抑制作用。[结论]柑橘皮油胞层中的活性物质具有较强的体外抗氧化活性和抑菌活性,甲醇是柑橘皮油胞层活性物质提取的较优提取剂。

    Abstract:

    [Objective ] To improve the deep processing and high-value utilization of citrus peels.[Methods ] The active components of the peel flavedo (C.aurantium var.daidai Tanaka,C.Kawachiensis and C.Sudachi ) were extracted with different solvents,the antioxidant activity was determined by DPPH and ABTS free radical scavenging rate,and the antibacterial activity was determined by inhibition zone,microbroth dilution method and antibacterial kinetics.[Results ] It was showed that the yield,active substance content,antioxidant activity and antibacterial activity of methanol extract were better than those of petroleum ether and n-hexane.The order of antioxidant activity of the extracts was C.Sudachi >C.aurantium var.daidai Tanaka >C.Kawachiensis,and the DPPH and ABTS free radical scavenging rates of methanol extract of C.Sudachi were 83.86% and 69.14%,respectively.Extracts could inhibit four foodborne pathogenic bacteria,among which had the most significant the inhibitory effect on Staphylococcus aureus (P<0.05).The order of antibacterial activity of flavedos extracts was C.Sudachi >C.aurantium var.daidai Tanaka >C.Kawachiensis,and the methanol extracts of C.Sudachi had the strongest antibacterial effects,of which the diameters of the inhibition zone against Escherichia coli,Salmonella and Staphylococcus aureus were 10~15 mm.[Conclusion ] The flavedo of citrus peel has strong in vitro antioxidant activity and antibacterial activity,methanol is the best extraction agent for the extraction of the flavedo ’s active substances.

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谭雨心,渡部由香,廖明系,等.柑橘皮油胞层提取物的抗氧化活性与抑菌活性[J].食品与机械,2024,40(9):145-152.
TAN Yuxin, YUKA Watanabe, LIAO Mingxi, et al. Study on antioxidant and antibacterial activity of extracts from flavedo of citrus peel[J]. Food & Machinery,2024,40(9):145-152.

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  • 收稿日期:2024-01-19
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  • 在线发布日期: 2025-02-18
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