基于Fe3O4磁分子印迹纳米粒子比色检测蔬菜中6-苄氨基腺嘌呤残留
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(1. 湖南农业大学食品科学技术学院,湖南 长沙 410128;2. 食品科学与生物技术湖南省重点实验室,湖南 长沙 410128)

作者简介:

刘艺丹,女,湖南农业大学在读硕士研究生。

通讯作者:

刘霞(1976—),女,湖南农业大学教授,博士。E-mail:liuxiaspr@aliyun.com

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基金项目:

国家自然科学基金项目(编号:31671931);湖南省自然科学基金(编号:2020JJ4354)


Colorimetric detection of 6-benzylaminopurine residues in vegetables based on Fe3O4 magnetic molecular-imprinted nanoparticles
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(1. School of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China;2. Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha, Hunan 410128, China)

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    摘要:

    [目的]实现蔬菜中6-苄氨基腺嘌呤(6-BA)残留的快速、灵敏、可视化检测。[方法]基于Fe3O4磁分子印迹纳米粒子(Fe3O4 MMIP NPs)的类过氧化物酶活性和特异识别性,构建了一种简单、快速、高选择性检测6-BA的比色方法,并用于蔬菜中6-BA残留检测。[结果]在pH 4.0、H2O2浓度0.02 mol/L、TMB浓度0.02 mol/L和反应时间5 min时,Fe3O4 MMIP NPs表现出最佳的类过氧化物酶催化活性。6-BA在1~300 ng/mL质量浓度范围内与空白和样品吸光度值的差值呈良好的线性关系,检测限为0.697 ng/mL。该方法被成功应用于蔬菜(黄瓜、西红柿、黄豆芽、绿豆芽)中6-BA残留检测,回收率为83.47%~106.76%,相对标准差为0.37%~5.66%。此外,4 ℃下,Fe3O4 MMIP NPs贮存60 d后仍能保持良好的催化活性。[结论]基于Fe3O4 MMIP NPs检测6-BA的比色方法具有简便、快速、经济等优势,能够用于蔬菜中6-BA残留检测,具备一定的可靠性和实用性。

    Abstract:

    [Objective] Achieving a rapid, sensitive, and visual detection of 6-benzylaminopurine (6-BA) residues in vegetables. [Methods] Based on the peroxidase-like activity and specific recognition of Fe3O4 magnetic molecularly imprinted nanoparticles (Fe3O4 MMIP NPs), a simple, rapid and highly selective colorimetric method was established to detect 6-BA residues in vegetables. [Results] Fe3O4 MMIP NPs exhibits optimal peroxidase-like catalytic activity at pH 4.0, 0.02 mol/L TMB, 0.02 mol/L H2O2 and a reaction time of 5 min. 6-BA showed a good linear relationship with the difference between the blank and the sample absorbance value in the mass concentration range of 1~300 ng/mL with a limit of detection at 0.697 ng/mL. The method was successfully applied to the detection of 6-BA residues in vegetables (cucumber, tomato, bean sprouts, mung bean sprouts). The recovery rate was 83.47%~106.76%, with a relative standard deviation of 0.37%~5.66%. In addition, Fe3O4 MMIP NPs could still maintain good catalytic activity after 60 days of storage at 4 ℃. [Conclusion] The colorimetric method for the detection of 6-BA based on Fe3O4 MMIP NPs has the advantages of simplicity, rapidity, economy, and can be used for the detection of 6-BA residues in vegetables, which has certain reliability and practicability.

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刘艺丹,栗 鑫,王婧丽,等.基于Fe3O4磁分子印迹纳米粒子比色检测蔬菜中6-苄氨基腺嘌呤残留[J].食品与机械,2024,40(6):69-76,103.
LIU Yidan, LI Xin, WANG Jingli, et al. Colorimetric detection of 6-benzylaminopurine residues in vegetables based on Fe3O4 magnetic molecular-imprinted nanoparticles[J]. Food & Machinery,2024,40(6):69-76,103.

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  • 收稿日期:2024-04-03
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  • 在线发布日期: 2024-07-22
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