Objective： This study aimed to explore the fatigue relieving effect of Lonicera caerulea Berry polyphenol, hawthorn polyphenol microcapsules, lactoferrin and vitamin C as polyphenol compound formula to alleviate fatigue. Methods： The commercial sports nutrition food with the compound formula of creatine, β-hydroxy-β-methylbutyrate, fructose diphosphate, taurine, vitamin B₆ and watermelon powder was used as the positive control. The experiment of exhaustive swimming in mice was carried out. The fatigue related indexes were determined by the kit, and the microscopic pathological tissues of the organs were observed by H&E staining. Results： There was no significant difference in body weight of mice (P>0.05). Compared with the normal group, no significant difference in organ index in the dietary supplement group (P>0.05) was found. Polyphenol compound formula significantly prolonged the swimming time of mice at room temperature and low temperature by 106.01% and 140.94%, respectively (P<0.01). Moreover, oxidative stress, metabolite accumulation and energy metabolism, and down-regulated secretion of inflammatory factors (P<0.01 or P<0.05) were also improved. It promoted the cell nucleus increase in muscle, inflammatory cell infiltration in the liver, and redness and shedding of tubular epithelial cells in the kidney. Conclusion： Polyphenol compound formula can relieve exercise fatigue at low temperature and room temperature.